FASTA sequence

Enter sequence

>sequence1 AGAGATTAGTCAGTACGGCTAGCTAGCTACGTCTATATTATAGCGATACGGG

or upload file

Motifs

Your motifs

>motif1 GGCTT >motif2 AAACGG

Options

Reverse complement sequence

Minimum spacing between motifs

Maximum spacing between motifs

Distance from left edge

Distance from righte edge

Interval of substitution of motifs

Barcode length

Minimum barcode GC content (%)

Maximum barcode GC content (%)

Barcode edit distance

Number of barcode per sequence