Guillermo Parada

PhD Student

 

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This person is a member of Sanger Institute Alumni.

The advent of high-throughput sequencing technologies is providing an unprecedented opportunity to explore the complexity of higher eukaryotes transcriptomes. 

My aim is to study the gene expression regulation mechanisms by analyzing high-throughput sequencing data.

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Non-canonical splice sites are highly involved in alternative splicing and some show tissue-specific alternative splicing patterns. (A) Participation of non-canonical splice sites in alternative splicing. (B) The use of a non-canonical splice site generates a frame shift and a premature termination codon that disrupts a NAP-like domain (highlighted in yellow) of the TSPYL2 protein. A tissue-specific pattern of 3′ alternative splice site selection is shown across seven human tissues. Coverage quantification is plotted, where error bars indicate the 95% binomial confidence interval. (C) CPSF3 gene has an alternative 5′-GG non-canonical splice site. Coverage quantification shows a testis-specific selection of the 5′-GG non-canonical splice site.

Non-canonical splice sites are highly involved in alternative splicing and some show tissue-specific alternative splicing patterns. (A) Participation of non-canonical splice sites in alternative splicing. (B) The use of a non-canonical splice site generates a frame shift and a premature termination codon that disrupts a NAP-like domain (highlighted in yellow) of the TSPYL2 protein. A tissue-specific pattern of 3′ alternative splice site selection is shown across seven human tissues. Coverage quantification is plotted, where error bars indicate the 95% binomial confidence interval. (C) CPSF3 gene has an alternative 5′-GG non-canonical splice site. Coverage quantification shows a testis-specific selection of the 5′-GG non-canonical splice site.

(A) Model of rat hsp70 gene based on our transcriptome profiling data analysis. (B) Whole brain transcriptome profiling data shows a predominant transcription start site at the beginning of the gene. (C) Cerebral cortex transcriptome profiling data shows predominant transcriptional start sites inside the codifying sequence of hsp70 gene. The coverage for reads aligned in the positive and negative strand are shown in red and blue, respectively.

(A) Model of rat hsp70 gene based on our transcriptome profiling data analysis. (B) Whole brain transcriptome profiling data shows a predominant transcription start site at the beginning of the gene. (C) Cerebral cortex transcriptome profiling data shows predominant transcriptional start sites inside the codifying sequence of hsp70 gene. The coverage for reads aligned in the positive and negative strand are shown in red and blue, respectively.